Table 3.
Antibodies for western blotting and immunofluorescence staining.
| Antibody | Manufacturer catalog# | Species | Dilution | |
|---|---|---|---|---|
| Western blotting | Immunofluorescence staining | |||
| F4/80 | Abcam, ab6640 | Rabbit | – | 1:100 |
| TLR4 | Abcam, ab13556 | Rabbit | 1:500 | – |
| ZO-1 | Thermo Fisher, 40-2200 | Rabbit | 1:500 | 1:100 |
| Occludin | Thermo Fisher, 40-4700 | Rabbit | 1:1,000 | 1:250 |
| Claudin-1 | Thermo Fisher, 71-7800 | Rabbit | 1:200 | 1:100 |
| MLC2 | Cell signaling Technology, 3672 | Rabbit | 1:1,000 | – |
| Phosphor-MLC2 (Ser19) | Cell signaling Technology, 3675 | Mouse | 1:1,000 | – |
| MYPT1 | Cell signaling Technology, 2634 | Rabbit | 1:1,000 | – |
| Phospho-MYPT1 (Thr696) | Cell Signaling Technology, 5163S | Rabbit | 1:1,000 | – |
| β-actin | Proteintech, 66009-1-lg | Mouse | 1:5,000 | – |
| Anti-rabbit IgG (DyLightTM 680 Conjugate) | Cell Signaling Technology, 5366 | Goat | 1:10,000 | – |
| Anti-mouse IgG (H+L) (DyLight 800 4X PEG Conjugate) | Cell Signaling Technology, 5257 | Goat | 1:10,000 | – |
| Anti-rabbit IgG H&L (Cy3®) | Abcam, ab6939 | Goat | – | 1:1,000 |
| Anti-rabbit IgG H&L (Alexa Fluor® 488) | Abcam, ab150077 | Goat | – | 1:1,000 |