Figure 3:

Identification of compounds that target DHFR to inhibit the growth of M. tuberculosis. A) 7 enriched chemotypes yielded 6 compounds with growth inhibitory activity and hypothesized to act through Rv2763c (Mtb DHFR). B) Correlation between in vitro inhibition of purified Mtb DHFR and inhibition of bacterial growth. Methotrexate (yellow) was used as a control for inhibiting the enzyme but having no efficacy against bacterial growth. Example class members are listed in the table, along with their IC₅₀ values for the Mtb enzyme, MITC95 values, the correlation between these, and their toxicity observed at 99μM in HeLa cells.