Figure 6.

α-Mangostin and mangosteen fruit extract, was incubated with gastrointestinal microsomes to mimic Phase I and II metabolism in the mouse or human GI tract. Human and mouse gastrointestinal microsomes were prepared as described in the materials and methods. Black bars represent the respective control for that individual time point. Gray bars represent the microsomal stability of α-Mangostin/MFE in the indicated enzyme system in both mouse and human gastrointestinal microsomes. A ‘no preincubation’ was performed (data not shown) and did not have any impact on the interpretation of this data. [A] α-Mangostin was incubated with mouse gastrointestinal microsomes (Phase 1); [B] α-Mangostin was incubated with human gastrointestinal microsomes (Phase 1); [C] α-Mangostin was incubated with mouse gastrointestinal microsomes (Phase 2); [D] α-Mangostin was incubated with human gastrointestinal microsomes (Phase 2); [E] Mangosteen Fruit Extract standardized to α-Mangostin was incubated with mouse gastrointestinal microsomes (Phase 1); [F] Mangosteen Fruit Extract standardized to α-Mangostin was incubated with human gastrointestinal microsomes (Phase 1); [G] Mangosteen Fruit Extract standardized to α-Mangostin was incubated with mouse gastrointestinal microsomes (Phase 2); [H] Mangosteen Fruit Extract standardized to α-Mangostin was incubated with human gastrointestinal microsomes (Phase 2);