Fig. 6.

Supraspinatus muscle fiber characterization. Male, female, and OVX fresh frozen muscle sections were stained for laminin (cell borders/red), MyHC type 2a (light gray/green), MyHC type 2b (dark gray/blue), MyHC type 2x (black/unstained), and MyHC type 1 fibers (not pictured) (a). Male fibers had increased average size compared to female fibers in deep (b) and superficial (c) muscle. There were no differences in regional distribution of fiber type across sex in deep (d) or superficial (e) muscle. Data represented as mean and standard deviation, n = 8/group. Significant differences are indicated by solid bars (p < 0.05/3) and trends are indicated by dashed lines (p < 0.1/3).