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. 2019 Jan;25(1):17–22. doi: 10.1261/rna.068593.118

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© 2019 Deryusheva and Gall; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — (A) A full-length human U87 scaRNA that contains a U6 snRNA insertion (U87-F-U6[48–70]) concentrates in CBs. (Left panel) Bright red FISH signals are detected in CBs of transfected HeLa cells after hybridization with a Cy3-labeled U87 antisense probe. (Middle panel) A few cells have a weak signal in the nucleoli. (Right panel) Control nontransfected cells show no detectable signal. Similar FISH patterns were observed when HeLa cells were transfected with U87-F constructs containing fragments of 18S and 28S rRNAs (not shown). (B) Fragments of rRNAs and U6 snRNA are properly modified when they are targeted to CBs (red traces). Modification also occurs when the fragments are inserted into a U87-S construct that lacks the H/ACA domain, which contains the CAB-box (blue trace). In each case, black traces are controls.