Fig 1. Fluorescent protein signal is proportional to gene copy number.

(A) Protein fluorescence increases with increasing copies of the mCitrine gene. We determined the fluorescence of haploid and diploid cells containing variable numbers of a constitutively expressed mCitrine gene integrated at either the HO locus and/or the dubious ORF, YLR123C. The two-copy diploid is heterozygous at both loci. Each distribution was estimated using 100,000 single-cell measurements normalized by forward scatter. (B) Schematic representation of how the fluorescent reporter enables CNV detection in heterogeneous evolving populations through quantitative changes in protein fluorescence. Data and computer code used to generate this figure can be accessed in OSF: https://osf.io/fxhze/. a.u., arbitrary units; CNV, copy number variant.