Table 2. Summary of single nucleotide variation in three different selection conditions.

Populations were sequenced at 150 and 250 generations. For variants that were identified at both time points, we determined whether they increased (↑) or decreased (↓) in frequency between generation 150 and 250.

	Glucose (n = 10)				Urea (n = 11)				Glutamine (n = 11)
	Total		Trend		Total		Trend		Total		Trend
Predicted effect	g150	g250	↑	↓	g150	g250	↑	↓	g150	g250	↑	↓
Noncoding	4	9	2	1	8	12	1	0	6	5	0	0
Missense	47	61	17	4	22	34	6	2	12	22	4	1
Frameshift	4	5	1	1	5	6	2	0	2	2	0	0
Synonymous	1	7	0	0	4	10	2	0	4	3	0	0
Stop gained	4	10	2	0	7	4	0	2	0	5	0	0
Start lost	0	1	0	0	0	0	0	0	0	0	0	0
Splice variant	2	3	0	1	0	0	0	0	0	1	0	0
Mito. genome	0	0	0	0	0	2	0	0	0	0	0	0
In-frame insertion	0	0	0	0	0	0	0	0	0	1	0	0
Total variants	62	96	22	7	46	68	11	4	24	39	4	1

Abbreviation: Mito., mitochondrial.