Table 4. Estimation of CNV lineages in evolving populations across time.

We determined the number of GAP1 CNV-containing lineages by correcting the number of identified barcodes by the estimated false positive rate associated with CNV isolation using FACS. High-confidence GAP1 CNV lineages are defined as those that are found at two or more consecutive time points. Data and computer code used to generate this table can be accessed in OSF: https://osf.io/fxhze/.

Population	Generation	Number of detected barcodes	FP	FP-corrected barcode count	Barcodes identified at >1 time point
bc01	70	9,650	0.27	7,067	891
bc01	90	1,064	0.09	973	891
bc01	150	136	0.04	131	131
bc01	270	79	0.04	76	38
bc02	70	7,243	0.27	5,305	2,676
bc02	90	5,851	0.09	5,351	2,710
bc02	150	606	0.04	583	162
bc02	270	29	0.04	28	22

Abbreviations: CNV, copy number variant; FACS, fluorescence-activated cell sorting; FP, false positive rate.