Fig 5. Gut microbiota disruption does not obviously affect the pulmonary resistance to S. pneumoniae in TLR4-deficient mice.

TLR4-deficient mice were treated with broad-spectrum antibiotics or autoclaved saline, and infected intra-nasally with 2.3 × 10⁸ CFU of S. pneumoniae. (A) The survival was observed for 7 days (n = 20 per group). (B) Bacterial load, and (C) TNF-α and (D) IL-1β concentration per mg lung tissue were determined 6 hours and 12 hours after infection (n = 6 per group). CFU, colony-forming units. Data are presented as means ± SD. Analyzed with the log-rank test or unpaired Student’s t test.