Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) |
Fgd5-CreERT2 | PMID:24958848 | RRID:IMSR_JAX:027789 | |
| Strain, strain background (M. musculus) |
Rosa26-rtTA;
Col1a1-tetO- H2B-mCherry |
PMID:17554301 | RRID:IMSR_JAX:014602 | |
| Strain, strain background (M. musculus) |
Rosa26-
Lox-Stop- Lox-Tomato |
PMID:20023653 | RRID:IMSR_JAX:007905 | |
| Antibody | B220 PECy5 | Biolegend | RRID:AB_312994 | (1:400) |
| Clone: RA3-6B2 | ||||
| Antibody | B220 biotin | Biolegend | RRID:AB_312989 | (1:200) |
| Clone: RA3-6B2 | ||||
| Antibody | B220 APC | Biolegend | RRID:AB_312997 | (1:400) |
| Clone: RA3-6B2 | ||||
| Antibody | CD105 PECy7 | Biolegend | RRID:AB_1027700 | (1:200) |
| Clone: MJ7/18 | ||||
| Antibody | CD115 BV605 | Biolegend | RRID:AB_2562760 | (1:200) |
| Clone: CSF-1R | ||||
| Antibody | CD11b PECy5 | Biolegend | RRID:AB_312793 | (1:400) |
| Clone: M1/70 | ||||
| Antibody | CD11b biotin | Biolegend | RRID:AB_312787 | (1:200) |
| Clone: M1/70 | ||||
| Antibody | CD11b APC | Biolegend | RRID:AB_312795 | (1:800) |
| Clone: M1/70 | ||||
| Antibody | CD11b APC-Cy7 | Biolegend | RRID:AB_830641 | (1:200) |
| Clone: M1/70 | ||||
| Antibody | CD11c BV570 | Biolegend | RRID:AB_10900261 | (1:200) |
| Clone: N418 | ||||
| Antibody | CD150 APC | Biolegend | RRID:AB_493460 | (1:400) |
| Clone: TC15-12F12.2 | ||||
| Antibody | CD150 PE | Biolegend | RRID:AB_313683 | (1:200) |
| Clone: TC15-12F12.2 | ||||
| Antibody | CD16/32 AL700 | eBioscience | RRID:AB_493994 | (1:100) |
| Clone: 93 | ||||
| Antibody | CD19 PECy7 | eBioscience | RRID:AB_657663 | (1:200) |
| Clone: 1D3 | ||||
| Antibody | CD19 BV-786 | BD-Horizon | RRID:AB_2738141 | (1:200) |
| Clone: 1D3 | ||||
| Antibody | CD25 APC | eBioscience | RRID:AB_469366 | (1:100) |
| Clone: PC 61.5 | ||||
| Antibody | CD31 PerCpCy5.5 | Biolegend | RRID:AB_2566761 | (1:200) |
| Clone: MEC13.3 | ||||
| Antibody | CD3e PECy5 | Biolegend | RRID:AB_312675 | (1:400) |
| Clone: 145–2 C11 | ||||
| Antibody | CD3e biotin | Biolegend | RRID:AB_2563947 | (1:200) |
| Clone: 17A2 | ||||
| Antibody | CD3 AL700 | Biolegend | RRID:AB_493697 | (1:200) |
| Clone: 17A2 | ||||
| Antibody | CD4 BV711 | Biolegend | RRID:AB_2562607 | (1:200) |
| Clone: RM4-5 | ||||
| Antibody | CD4 APC eFl780 | eBioscience | RRID:AB_1272219 | (1:200) |
| Clone: RM4-5 | ||||
| Antibody | CD41 PerCP-eFl710 | eBioscience | RRID:AB_10855042 | (1:200) |
| Clone: MWReg30 | ||||
| Antibody | CD45.1 AL700 | Biolegend | RRID:AB_493733 | (1:200) |
| Clone: A20 | ||||
| Antibody | CD45.2 PECy7 | Biolegend | RRID:AB_1186098 | (1:200) |
| Clone: 104 | ||||
| Antibody | CD45.2 PE/ Dazzle |
Biolegend | RRID:AB_2564177 | (1:200) |
| Clone: 104 | ||||
| Antibody | CD45.2 BV785 | Sony | RRID:AB_2562604 | (1:200) |
| Clone: 104 | ||||
| Antibody | CD45.2 biotin | Biolegend | RRID:AB_313441 | (1:200) |
| Clone: 104 | ||||
| Antibody | CD48 PECy7 | Biolegend | RRID:AB_2075049 | (1:200) |
| Clone: HM48-1 | ||||
| Antibody | CD48 AL700 | Biolegend | RRID:AB_10612755 | (1:200) |
| Clone: HM48-1 | ||||
| Antibody | CD5 BV-421 | BD-Horizon | RRID:AB_2737758 | (1:200) |
| Clone: 53–7.3 | ||||
| Antibody | CD8 PerCpCy5.5 | Sony | RRID:AB_2075239 | (1:200) |
| Clone: 53–6.7 | ||||
| Antibody | c-kit APCeFl780 | eBioscience | RRID:AB_1272177 | (1:200) |
| Clone: 2B8 | ||||
| Antibody | c-kit APC | Biolegend | RRID:AB_313221 | (1:100) |
| Clone: 2B8 | ||||
| Antibody | F4/80 BV421 | Biolegend | RRID:AB_11203717 | (1:200) |
| Clone: BM8 | ||||
| Antibody | Flt3 biotin | eBioscience | RRID:AB_466600 | (1:200) |
| Clone: AZF10 | ||||
| Antibody | Gr1 PECy5 | Biolegend | RRID:AB_313375 | (1:400) |
| Clone: RB6-8C5 | ||||
| Antibody | Gr1 FITC | BD PH | RRID:AB_394643 | (1:400) |
| Clone: RB6-8C5 | ||||
| Antibody | Gr1 biotin | Biolegend | RRID:AB_313369 | (1:200) |
| Clone: RB6-8C5 | ||||
| Antibody | Gr1 BV711 | Sony | RRID:AB_2562549 | (1:200) |
| Clone: RB6-8C5 | ||||
| Antibody | IL7Ra BV510 | Sony | RRID:AB_2564576 | (1:200) |
| Clone: A7R34 | ||||
| Antibody | Ly6G APC/Fire750 | Biolegend | RRID:AB_2616733 | (1:200) |
| Clone: 1A8 | ||||
| Antibody | NK1.1 PECy5 | Biolegend | RRID:AB_493591 | (1:400) |
| Clone: PK136 | ||||
| Antibody | NK1.1 Pacific Blue | Biolegend | RRID:AB_2132712 | (1:200) |
| Clone: PK136 | ||||
| Antibody | Sca1 Pacific Blue | Biolegend | RRID:AB_2143237 | (1:200) |
| Clone: E13-161.7 | ||||
| Antibody | TcR Vγ3 APC | Biolegend | RRID:AB_10895900 | (1:200) |
| Clone: 536 | ||||
| Antibody | TcR γ/δ BV605 | Biolegend | RRID:AB_2563356 | (1:200) |
| Clone: GL3 | ||||
| Antibody | Ter119 PECy5 | Biolegend | RRID:AB_313711 | (1:400) |
| Clone: Ter-119 | ||||
| Antibody | Ter119 biotin | Biolegend | RRID:AB_313705 | (1:200) |
| Clone: Ter-119 | ||||
| Antibody | Ter119 PerCpCy5.5 | Biolegend | RRID:AB_893636 | (1:200) |
| Clone: Ter-119 | ||||
| Peptide, recombinant protein |
Streptavidin BV605 |
Biolegend | (1:400) | |
| Peptide, recombinant protein |
Streptavidin- Saporin |
PMID: 27272386 Advanced Targeting Systems |
||
| Chemical compound, drug |
Tamoxifen | Sigma- Aldrich |
I.p. 50 mg/kg | |
| Chemical compound, drug |
Doxycycline | Ssniff Spezialdiäten |
Food 2 g/kg | |
| Chemical compound, drug |
Tamoxifen | Ssniff Spezialdiäten |
Food 400 mg/kg Tamoxifen Citrate |
|
| Software, algorithm |
Flowjo | FlowJo (https://www. flowjo.com/solutions/flowjo) |
RRID:S
CR_008520 |
|
| Software, algorithm |
Microsoft Excel |
Microsoft Excel (https://www.microsoft.com/en-gb/) |
RRID: SCR_016137 |
|
| Software, algorithm |
Graphpad Prism |
GraphPad Prism (https://graphpad.com) |
RRID: SCR_002798 |