Skip to main content
. 2018 Dec 12;7:e42179. doi: 10.7554/eLife.42179

Figure 2. S. cerevisiae and human mtRNAPs cap RNA with NAD+ and NADH more efficiently than bacterial and nuclear RNAPs.

(A and B) Dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for S. cerevisiae mtRNAP (A) and human mtRNAP (B) (mean ± SD; n = 3). DNA templates and representative data are shown in Figure 2—figure supplement 1. (C) Dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for mtRNAPs vs. E. coli RNAP and S. cerevisiae RNAP II. Top, tailed template. Grey box and arrow indicate TSS. Bottom, dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for S. cerevisiae mtRNAP (Sce mtRNAP), human mtRNAP, E. coli RNAP (Eco RNAP) and S. cerevisiae RNAP II (Sce RNAP II) (mean ± SD; n = 3).

Figure 2—source data 1. Data for Figure 2A,B.
elife-42179-fig2-data1.pdf (24KB, pdf)
DOI: 10.7554/eLife.42179.009
Figure 2—source data 2. Data for Figure 2C,D, and Figure 2—figure supplement 1.
elife-42179-fig2-data2.pdf (30.8KB, pdf)
DOI: 10.7554/eLife.42179.010

Figure 2.

Figure 2—figure supplement 1. Dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for mtRNAPs: representative data.

Figure 2—figure supplement 1.

(A and B) Panels show DNA templates and full-length RNA products of in vitro transcription reactions performed with S. cerevisiae mtRNAP (A) and human mtRNAP (B) with the indicated [NCIN] / [ATP] ratio. Grey box and arrow, TSS; +31, +17/18, +15/16, position of last NTP incorporated into RNA; M, 10-nt marker.
Figure 2—figure supplement 2. S. cerevisiae and human mtRNAPs cap RNA with NAD+ and NADH at least as efficiently as bacteriophage T7 RNAP.

Figure 2—figure supplement 2.

Dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for mtRNAPs vs. T7 RNAP. Top, tailed template. Grey box and arrow indicate TSS. Bottom, Dependence of NCIN-mediated capping with NAD+ and NADH on [NCIN] / [ATP] ratio for S. cerevisiae mtRNAP (Sce mtRNAP), human mtRNAP, and T7 RNAP (mean ± SD; n = 3).