Figure 5. Correlations between the peptide translocation time and cell size for 383 cells that were treated with an above-MIC concentration of 5-FAM-LC-LL37 peptides reported in Figure 2.

(A Left panel) A negative correlation between initial cell length and AMP translocation time indicates resistance of small cells to peptides until a later time points in their life cycle. (A Right panel) Cell length at translocation time and the time of translocation are not correlated. Average and standard deviation are depicted for the data binned based on the x axis. (B) Average cell size affects the MIC values. Addition of ${\displaystyle 1}$μM∕mL of cephalexin to the growth media (RDM) increases cell size by 17% (sample newborn cells shown in the bottom panel), which resulted in an increase in the MIC. The inoculum size of $6.1\times {10}^6$ cells/ml are used for this experiment. Twelve replicates with three biological repeats were used for this data.