Fig. 7.

MPP⁺ and GMF activate NF-κB in mouse mast cells, and mouse mast cell proteases activate NF-κB in astrocytes and glia-neurons. BMMCs were incubated with MPP⁺ (10 μM) or GMF (100 ng/ml), and astrocytes and glia-neurons were incubated with MMCP-6 or MMCP-7 for 24 h (a-c). Cell lysates were prepared from cell pellets and the expression of total and p-NF-κB p65 was determined by Western blot (N=3). The bands were visualized using ChemiDoc-It² Imaging System. Representative images show that MPP⁺, GMF and mast cell proteases-induced increased expression of p-NF-κB p65 in BMMCs (a), astrocytes (b) and glia-neurons (c)