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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Metab Brain Dis. 2018 Jun 18;33(5):1599–1607. doi: 10.1007/s11011-018-0266-7

Fig. 6. Upregulation of anti-inflammatory and regulatory chemokines and receptors in spinal cords of ABX mice.

Fig. 6

Chemokines and their receptors were measured in the spinal cords of mice after EAE. Since chemokines are known to attract specific types of immune cells a panel of chemokines and their receptors was used to look at their expression in the spinal cords of mice. cDNA from the spinal cords of mice were run on a RT2 Profiler™ PCR Array Mouse Chemokines & Receptors. Some of the results of the pooled samples were confirmed with RT-PCR on individual samples (n=3). Chemokines and receptors associated with anti-inflammatory microglia/macrophages were upregulated in ABX spinal cords, particularly CCR1 (p<0.001). Additionally, the chemokine CXCL12 (SDF-1α) which is associated with regulatory Th cells (CD4+ FoxP3+) was upregulated in ABX pretreated mice compared to control mice (p<0.001). Vertical dotted lines represent a threefold change in gene expression (threshold for fold difference). The solid gray line represents p=0.05, the cutoff for significance