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. Author manuscript; available in PMC: 2019 Dec 4.
Published in final edited form as: Circulation. 2018 Dec 4;138(23):2666–2681. doi: 10.1161/CIRCULATIONAHA.117.032273

Figure 2. Genome-editing of an isogenic corrected iPSC line using CRISPR.

Figure 2

(A) Schematic view of CRISPR/Cas9 and ssODN mediated genome editing. (B) Sequence of gRNA and ssODN for generating the isogenic corrected iPSC line. The blue capitalized ‘C’ annotates the desired Cytidine to be introduced to the VUSMYL3(170C>A) allele. The red lower-case letters display nucleotides positions of the VUSMYL3(170C>A), while the red capitalized ‘A’ represents a silent mutation. (C) Sanger sequencing confirmation of the isogenic corrected iPSC line (clone 1). Additional clones are presented in Supplemental Figure S3.