Figure 2.

Cardiac Functional and Histological Examinations

(A) Representative M-mode echocardiograms and Doppler spectra of mitral inflow in the sham-operated group (sham) and MI hamsters treated with vehicle (MI) or HA28 3 days after the operation. (B) Effects of HA28 on the LV ejection fraction (EF) and the ratio of the early (E) to late (A) ventricular filling velocities (E:A). HA28 at a dosage of 1, 3, or 10 mg/kg was subcutaneously injected 1 day before surgery and continued for 3 days thereafter. (C) Representative azan Mallory staining, toluidine blue staining, and chymase immunohistochemical staining of cardiac sections obtained 3 days after surgery. (D) Effects of HA28 on chymase-positive mast cells and cardiac chymase activities 3 days after MI. HA28 treatment was started 1 day before the induction of MI and continued for 3 days. Dd, left ventricular dimension end diastole; Ds, left ventricular dimension end systole; E, E-wave velocity; A, A-wave velocity. **p < 0.01 versus sham; #p < 0.05, ##p < 0.01 versus vehicle. Each group contained 6–8 animals. Significant differences among the mean values of multiple groups were evaluated with one-way ANOVA followed by post hoc analysis (Fisher’s test).