Fig. 3.

Calcium response in the cystic monolayer is triggered by high concentrations of ATP and consecutive ATP applications/washouts protocol do not cause P2 receptor desensitization. a Changes of the cystic cells’ intracellular calcium levels in response to various concentrations of ATP. Shown are representative transients from different cystic monolayers (12÷15 cells/ROIs were analyzed per each transient). b Representative calcium transients in response to three consecutive applications of ATP interleaved with 15-min long washout periods; 15 cells/ROIs were analyzed per each transient