Fig. 4.
Calcium transient in the cystic monolayer in response to P2X receptor agonist αβmeATP. a Representative changes of the cystic cells’ intracellular calcium levels in response to various concentrations of α,β-methylene-ATP (αβmeATP). Shown are representative transients from different cysts (12÷15 cells/ROIs were analyzed per each transient). b Response to ATP in a cystic monolayer is sustained after application of αβmeATP with 15-min-long washouts between applications of the compounds. N = 4 rats per group, 5÷8 cystic monolayers were tested in each group. c Summary for the amplitude and total [Ca2+]i production (AUC) for ATP and αβmeATP (200 μM each) (N ≥ 3 cyst monolayers for each group at selected concentration of agonist)