Fig. 5.
The effects of P2X antagonists isoPPADS, NF449 and AZ10606120 on calcium transients evoked by ATP in freshly isolated cystic epithelium. ATP was applied to establish the control level of the response (left), then, during the washout period, isoPPADS (a), NF449 (b), or AZ10606120 (c) were added to the bath solution, and incubation was performed (15 min); ATP was applied to the cystic monolayer in presence of the antagonist (middle). Following a second washout of all applied drugs (next 15 min), ATP was applied again to test the reversibility of the response (right). Shown are the representative responses from single cystic monolayers (11÷15 cells/ROIs were analyzed per transient). d The summary of the changes in the [Ca2+]i transient amplitude for the applied agonists/antagonists of purinergic signaling to the cystic epithelial cells (N = 3–8 cyst monolayers for each group)