Fig. 7.

Effects of intraperitoneal injection of PMA on total and plasma membrane protein levels of P2X3 receptor in L4, L5, and L6 DRGs of PDN rats subject to 2-Hz EA after EA treatment for 7 days. PMA was intraperitoneally injected (0.2 ng/μl, 100 μl) into the ventral surface of hind paw 10 min prior to EA treatment. Rats in the PDN + EA + PBS group received the same dose of PBS buffer as a control. a, b Representative Western blot protein and c relative amounts of total and plasma membrane P2X3 receptor in L4 DRGs. d, e Representative Western blot protein and f relative amounts of total and plasma membrane P2X3 receptor in L5 DRGs. g, h Representative Western blot protein and i relative amounts of total and plasma membrane P2X3 receptor in L6 DRGs. Results were expressed as relative fold changes as compared to the PDN + EA + PBS group after normalization to GAPDH. Data were presented as mean ± SD of three independent experiments, n = 5 per group. ^△ P < 0.05, ^△△ P < 0.01, compared with the PDN + EA + PBS group