Fig. 1.

CD73 expression in differently treated RPE cells. RPE cells were isolated from naïve and EAU-induced B6 mice. Amount of CD73 in membrane extract was determined by western blotting with Na⁺/K⁺ ATPase as reference (a). Naïve RPE cells were cultured, treated with LPS (50 ng/ml) and TNF-α (2000 ng/ml). Membrane CD73 was determined by western blot (b); Cd73 mRNA was detected by qPCR (c). a, b A representative western blotting result (left column) and optical density analysis (right column, n = 6). c qPCR determined Cd73 mRNA in freshly isolated RPE and cultured RPE (n = 6)