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. 2018 Dec 18;8:17924. doi: 10.1038/s41598-018-36394-0

Figure 7.

Figure 7

Effect of VGB4 on intracellular signaling pathways. (A) HUVECs were treated with VGB4 (0.55 and 0.74 μM) in the presence of VEGF-A (200 ng/ml) and then Cell lysates were subjected to western blot to analyze the expression level of p-ERK1/2, ERK1/2, p-AKT and AKT. GAPDH was used as reference to quantify protein bands by densitometry using ImageJ software. Full-length blots are presented in Supplementary Fig. S1. (B) The tumor tissues treated with VGB4 on the last day of the peptide administration (day 28) (5 and 10 mg/kg) were subjected to western blot to analyze the expression level of p-ERK1/2, ERK1/2, p-AKT and AKT. (C) 4T1 tumor tissue sections treated with VGB4 (10 mg/kg) were subjected to western blot to analyze the expression level of NF-κB, E-cadherin, N-cadherin and MMP-9. GAPDH was used as reference to quantify the protein bands by densitometry using ImageJ software. (B) and (C) Full-length blots are presented in Supplementary Fig. S2. All data were expressed as mean ± SD, n = 3, *P ≤ 0.05, **P ≤ 0.001 compared to the control.