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. 2018 Dec 18;8:17922. doi: 10.1038/s41598-018-36174-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Least square means of D. melanogaster developmental time (DT) across generations and across breeding intervals within generations. On the X axis each number denotes a consecutive day on which the eggs were laid by the same parents. The parents were approximately 6 days post eclosion old when they began to breed. Whiskers represent the standard error of the mean.