Table 2.
Genotoxicity of E171 in D. melanogaster third instar larvae in F1, F10 and F20 generation.
| Comet class | Total comet score | |||||
|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | ||
| EMS | 40.4 ± 1.2 | 34.6 ± 0.8 | 17.3 ± 2.3 | 5.8 ± 0.6 | 1.9 ± 0.00 | 94.2 ± 1.3bcdefg |
| F1 generation | ||||||
| Negative control | 89.5 ± 0.71 | 10.5 ± 1.7 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 10.5 ± 1.7ac |
| E171 | 85.8 ± 5.1 | 10.4 ± 3.4 | 3.8 ± 2.5 | 0.00 ± 0.00 | 0.00 ± 0.00 | 18 ± 0.8abcdfg |
| F10 generation | ||||||
| Negative control | 91.9 ± 1.1 | 8.1 ± 1.2 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 8.1 ± 1.2abd |
| E171 | 86.8 ± 0.7 | 6 ± 3.1 | 7.2 ± 2.9 | 0.00 ± 0.00 | 0.00 ± 0.00 | 20.4 ± 1.5abcdeg |
| F20 generation | ||||||
| Negative control | 92.8 ± 0.52 | 4.3 ± 0.31 | 2.9 ± 0.7 | 0.00 ± 0.00 | 0.00 ± 0.00 | 10.1 ± 0.54ac |
| E171 | 83.7 ± 0.8 | 7.2 ± 1.01 | 9.1 ± 0.5 | 0.00 ± 0.00 | 0.00 ± 0.00 | 25.4 ± 0.83abcdef |
ap < 0.05 when compared with the positive control group.
bp < 0.05 when compared with the negative control group in the F1 generation.
cp < 0.05 when compared with the negative control group in the F10 generation.
dp < 0.05 when compared with the negative control group in the F20 generation.
ep < 0.05 when compared with the E171 in the F1 generation.
fp < 0.05 when compared with the E171 in the F10 generation.
gp < 0.05 when compared with the E171 in the F20 generation.