Figure 10.

Elimination of ¹O₂, ONOO⁻, and ONOOH prevents viral RNA damage of FCV by the DBD plasma torch. FCV-infected cell lysates plus the indicated concentrations of radical scavengers for ¹O₂ (NaN₃) (a,d), ONOO⁻ (uric acid) (b,e), and ONOOH (ascorbic acid) (c,f) were subjected to DBD plasma torch treatment for 2 min. Viral RNA was extracted and the levels of intact viral RNA were analyzed by real-time polymerase chain reaction (PCR) using primers for FCV [(a–c) FCV-F1 and FCV-R1 or (d–f) FCV-F2 and FCV-R2] as described in Materials and Methods. Differences with **p < 0.01 or *p < 0.05 versus the control (0 mM for NaN₃ and ascorbic acid; 0 µM for uric acid) were considered significant; NS means no significance.