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. 2018 Dec 18;8:17947. doi: 10.1038/s41598-018-36779-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability of FCV under various conditions. After heat treatment (35–68 °C) for 2 min (a), UV-A treatment at the indicated energy (mJ/cm²) for the indicated time (0–30 min) using a UVGL-58 device (UVP) (b), treatment with peroxynitrite (ONOO⁻) (0–8 mM) for 2 min (c), treatment with hydrogen peroxide (H₂O₂) (0–3%) for 2 min (d), the FCV-infected cell lysate was subjected to viral titration assay to determine viral titer (TCID₅₀/ml). Refer to Materials and Methods for detailed descriptions of the protocols. Zero in virus titer means below the detectable limit. Differences where *p < 0.05 and **p < 0.01 versus control (35 °C for temperature, 0 mJ/cm² for UV-A, 0 mM for ONOO⁻, and 0% for H₂O₂) were considered significant, while NS means no significance.