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. 2018 Dec 18;8:17949. doi: 10.1038/s41598-018-36381-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HIF-1α measurement in hypoxic-induced cell lines. Three breast cell lines; (A) MDA-MB-231, (B) MCF-7, and (C) MCF-10A, were harvested after 1-, 3-, 5-days under normoxic (21% O₂) and hypoxic (1% and 3% O₂) conditions. Normalized HIF-1α levels over total protein levels. (D–F) HIF-1α knock-down using siRNA. Corresponding bar graphs represent relative HIF-1α normalized to the respective control cells in hypoxic condition. Data are presented as the mean ± SEM of duplicate independent samples. **p < 0.01 versus hypoxia-treated cells; n = 2 due to consistent datasets.