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. 2018 Dec 12;9:1441. doi: 10.3389/fphar.2018.01441

FIGURE 3.

FIGURE 3

Effect of ZCO on IL-4 production in RBL-2H3 cells. (A) RBL-2H3 cells were sensitized with IgE overnight at 37°C, followed by pretreatment with ZCO (0.0025, 0.005, and 0.01%) or quercetin (20 μM) for 2 h before stimulation with 1 μg/mL DNP-BSA for 16 h. The supernatants were analyzed for IL-4 production. Experiments were conducted in quadruplicate and expressed as mean ± SEM (∗∗∗P < 0.001 versus with DNP-BSA group). (B) RBL-2H3 cells incubated into 48-well plate overnight were pretreated with ZCO or quercetin for 2 h prior to stimulation with 30 ng/mL of PMA plus 350 ng/mL of A23187 for 16 h. The supernatants were collected and IL-4 cytokine was tested by using ELISA kit (∗∗∗P < 0.001 versus with PMA/A23187 group). +++means the significance compared with the mock cells (deal with nothing).