Fig. 3.
Inhibition of cyt-bc1:aa3 arrests oxidative phosphorylation in classical M. ulcerans. a Oxygen consumption rates in the classical lineage M. ulcerans strain S1013 treated with Q203 (5 nM, red squares), ND-11176 (50 nM, purple diamonds), streptomycin (5000 nM, green triangles) or DMSO (1%, black circles) was measured using the MitoXpress® Xtra-oxygen probe. Fluorescence (Ex380 nm, Em650 nm) was measured over 350 min. The experiment was performed in triplicate and repeated two times. b Effect of Q203 (5 nM) or streptomycin (5000 nM) treatment on the oxygen consumption rate in the classical strain S1013 (dark grey bars) and the ancestral strain S1325. c, d Quantification of intracellular ATP levels in the classical strain S1013 (c) or in the ancestral strain S1325 (d) treated with a dose range of Q203, ND-11176 or bedaquiline (BDQ) for 24 h. Every concentration was tested in triplicate and the assay repeated once