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. 2018 Dec 18;4:115. doi: 10.1038/s41420-018-0135-5

Fig. 4. Thapsigargin-induced UPR is diminished in SEC24A mutants.

Fig. 4

CHOP and ATF4 levels were used as indicators of UPR activation in HAP1 WT and representative SEC24A mutants 1 and 2 after an 18 h treatment with thapsigargin (TG; 0.062 µg/mL), tunicamycin (TN; 0.2 µg/mL), or brefeldin A (BFA; 0.045 µg/mL). a Western blot of CHOP and ATF4 as indicators of the UPR in HAP1 WT cells and SEC24A mutants with and without ER stress induction. b Quantification of CHOP and ATF4 levels in SEC24A mutants relative to HAP1 WT cells for each treatment. Levels are expressed as percentages, where 100% (dotted line) represents CHOP or ATF4 levels in treated HAP1 WT cells