Table 1.
Sequences of primers and oligonucleotides
| Identification | Sequence (5’→3’) |
|---|---|
| Amplification primers for deep sequencing | |
| PCR1 forward primer | AATGGACTATCATTGAAAGTATTTCG |
| PCR1 reverse primer | TCTACTATTCTTTGATGTGCGCTCTG |
| PCR3 forward primer | AATGATACGGCGACCACCGAGATC |
| PCR3 reverse primer | CAAGCAGAAGACGGCATACGAGAT |
| U6 forward primer | CGTGACGTAGAAAGTAATAATTTCTTGGG |
| sgRNA sequences | |
| MFSD2A sgRNA 1 | GAACATGGTGAGAGCCGAGT |
| MFSD2A sgRNA 1 | GGAAACAAGGCGTGTCTGCT |
| ARF sgRNA 1 | TCTGCTTCTTGCCAAATAGT |
| ARF sgRNA 2 | CTAGTTGGATTGGATGCTGC |
| SEC24A sgRNA 1 | AGTAGTTACGACGAGATTGA |
| SEC24A sgRNA 2 | GCAGGACCACTATGAAGCGA |
| SEC24A sgRNA 3 | GGGATGATGCACGAGGACAA |
| PNPLA8 sgRNA 1 | ATCCGAATTCTCTCAATTGA |
| EGFP sgRNA | AGCTGGACGGCGACGTAAA |
| Amplification primers for Sanger sequencing | |
| SEC24A forward primer | CAAGGATACAATTTCCAGCTTCCAG |
| SEC24A reverse primer | TATATTGCCAATTCAAAGGTGGTGG |
| PNPLA8 forward primer | TCAGGCTGTCAGCAAAGACA |
| PNPLA8 reverse primer | AGCTTTGGGTGTTATCCCTCT |