Fig. 3.

Differential gene expression between LSMMG and gravity (G) in a ∆hfq mutant at 12 h. a Volcano plot visualizing the global transcriptional changes within the mutant cells. All normalized transcripts were plotted and each point reflects one gene with those in red indicating significance (adjusted p-value < 0.05). Full details of the significant differentially expressed genes are listed in Supplemental Table S1. b Quantitative real-time PCR of selected genes compared under LSMMG and gravity conditions in WT and ∆hfq mutants. Genes selected were significant in the RNA-seq results and included genes encoding a flagellin structural protein (flaA), flagellar hook-associated protein (flgK), and catalase (katA). Asterisk indicates significance between comparisons and error bars reflect the standard error of the mean