Table 2.
General features of mobile DNA found in Dehalococcoides mccartyi targeted by CRISPR-Cas system
| Type | Prophage/phage | Integrative and mobilizable elements (IMEs) | ||
|---|---|---|---|---|
| Class | Prophage/phage | IME1 | IME with rdhA gene | IME2 |
| Size (kbp) | 6–40 | 21–23 | 4–11 | 8–9 |
| GC content | 49% | 44% | 41% | 38% |
| Integration sites | At ~800 and ~300 kbp | tRNA-Ala, Ile or lys | Multiple tRNA or tmRNA | At ~780 kbp |
| Naming convention | strain name - number | IME1-number | IME-rdhA present | IME2-number |
| General description | Siphoroviridae type virus with lytic/lysogenic cycle. | Contain phage-like integration and excision machinery. Observed to replicate as a high copy circular element or integrated within genome. | Contain phage-like integration and excision machinery. Observed to replicate as a high copy circular element or integrated within genome. Carry a reductive dehalogenase (rdhA) gene. | Predicted genomic island. Targeted by CRISPR function unknown |