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. 2018 Dec 12;9:3080. doi: 10.3389/fmicb.2018.03080

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Copyright © 2018 Zhao, Zhu, Guo and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the general effects of 3′ UTRs on mRNA expression in Yersinia pestis. (A,B) qRT-PCR analysis of transcripts of genes containing AT-rich (A) or normal (B) 3′ IGRs in the wild type (WT) and nucleotides 1–300 of the 3′ terminus (3′ T_1-300) mutant strains (Δ3′T_1-300), in which the 3′ T_1-300 is disrupted by insertion of a kanamycin (kan) cassette. The Y. pestis WT strain was used as a control, and the fold change in relative mRNA level for each strain was compared with that of the WT (set as 1). Student’s t-test was used to calculate p-values. ^∗p < 0.05, ^∗∗p < 0.001. Means ± standard deviation (SD) from three independent experiments are shown.