FIGURE 2.

The effects of 3′ UTRs on expression of a heterologous green fluorescent (GFP) reporter. (A) Schematic representation of GFP reporter with different 3′ UTRs and an rrnB transcriptional terminator. (B) Relative GFP fluorescence of E. coli cells expressing GFP with different 3′ UTRs (GFP-3′T_1-300) and the rrnB terminator, and without an additional 3′ UTR (pAcGFP1). Relative fluorescence intensities were normalized against the cell density (OD₆₀₀ value) of the bacterial culture, and the fold change in fluorescence was compared with that of the vector control (set as 1). Student’s t-tests were used to calculate p-values. ^∗p < 0.01. Means ± SD from three independent experiments are indicated.