Fig. 1.

a) Normalized spectra of the fluorescence of ADIFAB in measuring buffer at different pH: 6.2 (black line), 6.4 (red), 6.6 (green), 7.0 (yellow), 7.2 (blue), 7.4 (purple), 7.6 (cyan). All spectra were acquired at 20 °C. b) Difference spectra obtained by subtraction of the spectrum at reference pH (7.4, pH of the standard measuring buffer for ADIFAB). Color code as in a).