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PCR detection of periodontal disease-related bacteria on the tongue surface. PCR was performed to detect T. denticola, T. forsythia and P. gingivalis DNA. 10 µl of each 20-µl PCR product was separated on a 2% agarose gel. P. gingivalis, Porphyromonas gingivalis; T. forsythia, Tannerella forsythia; T. denticola, Treponema denticola; PCR, polymerase chain reaction.
