FIG 1.

Clotting time of human plasma is increased when incubated with culture supernatant from A. baumannii but not from strains deficient in either CpaA or the T2SS. An aPTT assay was performed after incubation of normal human plasma with culture supernatant from A. baumannii strain AB031, AB031 ΔcpaA, or AB031 ΔgspD (A), AB031 or ΔcpaA with either empty vector pMMB960 (p), pcpaA or pcpaAB (B), AB031 or ΔgspD with either empty vector pMMB67 (p) or pgspD (C), and A. baumannii strain 17978 or the T2SS mutant 17978 ΔgspD with either empty vector pMMB67 (p) or pcpaAB (D). The growth medium LB served as a negative control. Values that are significantly different are indicated by ordinary one-way ANOVA (n = 6) are indicated by bars and asterisks as follows: ***, P ≤ 0.001; **, P ≤ 0.01.