FIG 4.

DG167 synergizes with INH in vitro and in vivo. (A) In vitro killing curves for M. tuberculosis strain H37Rv after incubation with given concentrations of DG167 or INH or a combination of those drugs. Killing activity was monitored by plating for CFU. (B) Venn diagram showing differential gene expression data from an RNA-seq experiment upon treatment with 10× DG167 or 10× INH or the drug combination. (C) In vivo efficacy of DG167 and INH alone and in combination in an acute model of M. tuberculosis infection in mice. The arrow indicates the day when treatment was started. The significance was determined using the Kruskal-Wallis test. On days 21 and 28, all treatments showed significant effects compared to the control (P = <0.0001).