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Mouse spermatozoa extracted from vas deferens that were sonicated prior to fixation in 2% formaldehyde, without permeabilization. (A) DAPI alone. (B) Antibody alone. (C) Merge A and B. Note that under these preparatory conditions the PAS (white arrow) is immunoreactive to the antibody but the perforatorium is not labeled. Blue = DAPI, Green = anti-WBP2 (N-14), bar = 5 μm.
