Figure 3.

Immunocytochemistry showing specificity of WBP2 labeling in mature mouse spermatozoa. Mouse spermatozoa from the vas deferens were sonicated for 5 s, fixed in 4% paraformaldehyde and permeabilized with Triton-X-100. (A) DAPI alone. (B) Antibody alone. (C) Merge A and B. Note in B and C that with the added permeabilization step the perforatorium became immunoreactive (white arrows) in addition to the PAS (compare with Figure 2). (D) When the anti-WBP2 antibody was pre-incubated with its blocking peptide before primary antibody incubation no labeling was found in the PT regions. Blue = DAPI, Red = anti-WBP2 (N-14), bar = 5 μm.