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. 2018 Jul 11;99(6):1171–1183. doi: 10.1093/biolre/ioy156

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© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Immunocytochemistry showing the presence of WPB2 in elongating and elongated mouse spermatids fixed with 2% formaldehyde. (A) DAPI alone. (B) Antibody alone. (C) Merge A and B. (D) Merge C with E, which is a differential interference contrast picture of the same field. Note that in the elongated spermatid, at the top of the panel, WBP2 has been incorporated as part of the PAS while in the elongating spermatid, found below, WBP2 is still distributed throughout the cytoplasm lobe. Blue = DAPI, Green = anti-WBP2 (N-14), bar = 5 μm.