Figure 7.

Immunofluorescence showing developmental progression, during the 16 steps of spermiogenesis, in and the association of WBP2 and Tubulin (manchette) in mouse spermatids. Mouse spermatids from testicular extract were fixed in 4% paraformaldehyde and permeabilized after fixation with Triton-X-100. (A) DAPI alone. (B) Anti-WBP2 antibody alone. (C) Merge A and B. (D) Anti-Tubulin antibody alone. (E) C and D merged. Blue = DAPI, Red = anti-WBP2 (N-14), Green = anti-Tubulin, Yellow = the colocalization of WBP2 and Tubulin, Bar = 5 μm.