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. 2018 Dec 19;8:17969. doi: 10.1038/s41598-018-36156-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular and western blot analyses for confirmation of transformation of hairy root lines. (A) WT hairy roots infected with Agrobacterium rhizogenes C58C1. Transgenic hairy root lines infected with A. rhizogenes C58C1 harboring the binary vector pCAMBIA1301-GFP (B), pVHb (C), pVHb-AtHRS1 (D), pVHb-CoxIV (E), and pVHb-RecA (F). The numbers indicate the hairy root lines. (M) Molecular size marker (2 kb ladder). (P) The corresponding engineered bacteria (positive control). (N) WT hairy root (negative control). (G) Results of western blot analysis showing the expression of VHb, detected using a GFP-specific monoclonal antibody in non-transgenic and four independent transgenic hairy root lines.