Table 1.

Gene constructs and derived root cultures.

T-DNA construct	Number of established root lines			Established root cultures
	total	Antibiotic-resistant	PCR-positive
<AtHRS1 < VHb < hpt	22	9	8	A3, A7, A8, A11, A12, A15, A18, A21
<CoxIV < VHb < hpt	20	10	8	C3, C7, C9, C10, C13, C16, C18, C20
<RecA < VHb < hpt	20	9	8	R4, R6, R8, R11, R13, R15, R17, R20
<VHb < hpt	25	11	8	V3, V6, V9, V10, V13, V15, V19, V20

T-DNA, portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells. The antibiotic resistance gene (hpt) is always placed near the left border of the T-DNA. In addition, seven transformed with C58C1 were also established, as WT control.