Table 2.
Isolate ID | Type of Sample | lytA of OP specimen | Species isolated | Optochin | lytA of S. mitis isolate | Immunochro-matographic testing (BinaxNow) | Serotyping assays | Capsule visualization by Quellung using pneumococcal anti-serum | ||
---|---|---|---|---|---|---|---|---|---|---|
Real-time PCR | Conventional PCR | Latex agglutination | ||||||||
L006 | OP | Negative | S. mitis | Resistant | Negative | Negative | Serotype 1 | Serotype 1 | Serotype 1 | Weak visualization of capsule with serotype 1 anti-serum |
L115 | OP | Negative | S. mitis | Resistant | Negative | Positive | Serotype 1 | Serotype 1 | Serotype 1 (weak reaction) | No visualization |
L116 | OP | Negative | S. mitis | Resistant | Negative | Positive | Serotype 1 | Serotype 1 | Serotype 1 (weak reaction) | No visualization |
L121 | OP | Positive (Ct = 31.19) | S. mitis | Resistant | Negative | Negative | Serotype 1 | Serotype 1 | Serotype 1 | Weak visualization of capsule with serotype 1 anti-serum |
L164 | OP | Positive (Ct = 28.98) | S. mitis | Resistant | Negative | Negative | Serotype 1 | Serotype 1 | Serotype 1 (weak reaction) | Weak visualization of capsule with serotype 1 anti-serum |
Ct = cycle threshold.