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. 2018 Dec 19;8:17979. doi: 10.1038/s41598-018-36365-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of different maneuvers on the activation of myeloid cells. (A) Representative flow cytometry plot of WBCs stained with anti-CD11b and anti-CD16/32 in control, IPC, RIPer and IPC + RIPer group. (B) Percentages of CD11b+ cells in WBCs. (C,D) Percentages of CD16/32+ cells and CD16/32high in CD11b+ cells. *p < 0.05; **p < 0.005; ***p < 0.0005 vs control group, paired Student t-test. ^#p < 0.05; ^##p < 0.005; ^###p < 0.0005 vs different conditioning method, one-way ANOVA and Bonferroni post-hoc test.