Figure 1.

Neutrophils migrates to tumor cells. Purified neutrophils from healthy adults migrating to tumor cells were observed under inverted phase-contrast microscope (a) and the progress was monitored by using living cell station (b), scar bar, 200 μm. Neutrophils chemotaxis toward A549 cells was assayed by using under agarosechemotaxis model or transwell model. In under agarosechemotaxis model, the results were presented by the migration distance after 2 hours (c, d). In transwell model, cells were counted from the bottom of transwell plate after 2 hours (e). The production of IL-8, GROα, ENA-78 by A549 cell lines was measured by proteome array (f, g). a=medium; b=tumor supernatant; c=tumor; d= IL-8. Data are representative of four (a, d, e) or three (b, f, g) independent experiments. Mean and SD are presented. ***P<0.001, compared with control group (ANOVA with Tukey's test).