Figure 4.

Neutrophils arrest cell cycle of tumor cells. Neutrophils co-incubated with A549 cells at E:T ratios of 20:1, 10:1 and 3:1. Using cell cycle rapid solution to determine the cell cycle distribution, which was analyzed by flow cytometry. The representative images and detail data were shown in a, b. The related cell cycle regulation proteins were evaluated by western blot (c,d). Data are representative of four independent experiments. Mean and SD are presented. Statistical analysis was performed by one way ANOVA; ***P<0.001, compared with control group.