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. 2018 Nov 28;14(14):2103–2113. doi: 10.7150/ijbs.29297

Figure 5.

Figure 5

Neutrophils arrest cell cycle of tumor cells via Fas/ Fas ligand pathway. A549 cancer cells co-incubated with human neutrophils from healthy donors in the presence or absence of the Fas antagonist (BMS140) and agonist (EOS9.1) at effector to target (E:T) cell ratio of 20:1. Cell cycle and apoptosis of A549 cells were detected by flow cytometry (d, e). Fas L and Fas expression level were further examined by using flow cytometry (c). The relevant cell cycle regulation proteins were evaluated by western blot (f, g). Fas was knock out in A549 cells. The relevant empty lentivectors were used to induce control nonspecific (NS) expressing cells. Expression of Fas in knock out A549 cells was detected by western blot (a, b). Knock out tumor cells (A549, A431) co-incubated with neutrophils for 24 hours at E:T ratio of 20:1, cell cycle and apoptosis of tumor cells were examined (d, e). Data are representative of four independent experiments. Mean and SD are presented. Statistical analysis was performed by one way ANOVA; ***P<0.001, compared with control group.